Russian botanist Tswett invented chromatography as a separation technique. He describes in detail the separation of pigments, the coloured substances by filtration through column, followed by developments with pure solvents. The aim of this research works is to develop a novel, accurate, precise,cost – effective analytical method by using RP-HPLC method , for the selected immunosuppressive agent (voclosporin). Voclosporin is a calcineurin inhibitor for the treatment of lupus nephritis (LN) in patients diagnosed with systemic lupus erythematosus (SLE). Based up on the solubility of the drugs, diluent was selected, Acetonitrile and Water taken in the ratio of 50:50. A Standard solution of Voclosporin working standard was prepared as per procedure and was injected five times into the HPLC system. Chromatographic conditions used are stationary phase Kromasil (250mm*4.6mm 5?), Mobile phase0.01N Kh2po4 Buffer: Acetonitrilein the ratio of 60:40 and flow ratewas maintained at 1ml/min, detection wave length was282nm, column temperature was set to30oCand diluent was mobile phaseConditions were finalized as optimized method

In the present work, floating microspheres of Terfenadine using Chitosan, Eudragt S 100, Eudrgit L100 as copolymers were formulated to deliver Terfenadine via oral route. The results of this investigation indicate that ionic crosslinking technique Ionotropic gelation method can be successfully employed to fabricate Terfenadine microspheres. The technique provides characteristic advantage over conventional microsphere method, which involves an “all-aqueous” system, avoids residual solvents in microspheres. Other methods utilize larger volume of organic solvents, which are costly and hazardous because of the possible explosion, air pollution, toxicity and difficult to remove traces of organic solvent completely. Micromeritic studies revealed that the mean particle size of the prepared microspheres was in the size range of 563-883µm and are suitable for bio adhesive microspheres for oral administration. Increase in the polymer concentration led to increase in % Yield, % Drug entrapment efficiency, Particle size, % swelling and % Mucoadhesion. Based on the in-vitro dissolution studies T4 formulation prepared with Chitosan 250 mg was considered as the best formulation since it has shown the maximum drug release of 97.15% in 12hrs compared to all other formulations

Superporous hydrogels (SPHs) is originally developed as a novel drug delivery system to retain drugs in the gastric medium by instant swelling on water absorption through open porous structure and maintain their integrity in that harsh environment. Propranolol Hydrocloride, an antihypertensive drug with a short half-life, limited bioavailability, unstable nature at basic pH potentiated the need for developing a gastro-retentive system, hence super porous hydrogel of Propranolol Hydrocloride had been developed with cellulosic polymers, and adequate strength was imparted by the addition of Acidic Solution. The structural morphology of hydrogel was investigated by SEM, and it was found that plenty of pores of different size ranges, like 1 ?m, 2 ?m, 10?m were formed. Compatibility studies proved the integrity of the super porous hydrogel. Gelation time was found to vary with respect to the formulation. The setting time of super porous hydrogel was found to be increased with an increase in the concentration of HPMC K100M. The drug release from super porous hydrogels was sustained for 10 h. In-vitro drug release data obtained were fitted into various kinetic equations. The formulations obeyed Higuchi and Korsmeyer- Peppas kinetics of drug release. For further confirmation, the data were fitted to the Kopcha model to get the evidence of drug release by the combination of diffusion-controlled and chain relaxation–swelling mechanism. However, the diffusion mechanism predominated the process leading to quasi diffusion and anomalous diffusion mechanism

Bepotastine besilate is an effective second-generation antihistamine for treating allergic rhinitis and chronic urticaria. However, its short half-life necessitates frequent dosing, decreasing patient compliance. A floating tablet formulation of bepotastine besilate was developed and evaluated with the goal of prolonging gastric retention and improving therapeutic efficacy. A variety of polymers, including hydroxypropyl methylcellulose (HPMC), xanthan gum, and carbopol, as well as effervescent agents like sodium bicarbonate and citric acid, were used to prepare floating tablets using the direct compression method. In preformulation studies, bepotastine besilate was found to be compatible with the selected polymers and excipients. A variety of physical properties were evaluated for the floating tablets, including hardness, friability, weight variation, and uniformity of drug content. FTIR analyses have confirmed the stability of bepotastine besilate within the polymer matrix and the absence of significant drugpolymer interactions. Floating tablets of bepotastine besilate developed in this study may improve patient compliance by reducing dosing frequency and ensuring sustained therapeutic effects

The purpose of this study was to formulate and evaluate a self-microemulsifying drug delivery system (SMEDDS) for Captopril, an antihypertensive drug with low bioavailability due to poor water solubility. The primary aim was to enhance the solubility and oral bioavailability of Captopril by developing an optimized SMEDDS formulation. The formulation process involved selecting appropriate oils, surfactants, and co-surfactants based on their emulsification efficiency and compatibility with Captopril. The selected formulations were evaluated for various physicochemical parameters, including droplet size, polydispersity index (PDI), zeta potential, and self-emulsification time. The self-emulsification time was found to be less than 60 seconds, confirming the rapid formation of a microemulsion. In vitro drug release studies demonstrated a significantly enhanced dissolution rate of Captopril from the SMEDDS compared to the pure drug and conventional formulations. The study concluded that the SMEDDS formulation successfully improved the solubility and dissolution rate of Captopril, suggesting its potential to enhance the drug's oral bioavailability. Further in vivo studies are recommended to evaluate the pharmacokinetic parameters and therapeutic efficacy of the developed SMEDDS