ABSTRACT

A simple, accurate and reproducible RP-HPLC method has been developed for the determination of Lamivudine in tablet dosage form and in liquid dosage form. Chromatography was carried out on a Thermo Hypersil ODS-C18 column using a mobile phase consisting of and water acetonitrile (3:7 v/v) at a flow rate of 0.8 ml/min. The detection was made at 270 nm. The retention times for Lamivudine, was found to be 1.3 min. The calibration curves were linear over the range 10-120 μg/ml for Lamivudine. The proposed method was validated as per ICH guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet as well as dosage forms.

Keywords: Lamivudine, RP-HPLC, Tablet, Liquid form.

ABSTRACT

In the present study, Valacyclovir tablets were formulated using different formulation parameters and their effects on the dissolution rate of these tablets were evaluated. The binding agent used in this study was Povidone. Tablets were prepared by wet granulation method and were evaluated for various parameters like weight variation, hardness, friability, disintegration time, drug content and in vitro dissolution studies. This Process validation report for Valacyclovir Hydrochloride Tablets 500mg & 1gram common blend is based on the observations/data collected during manufacturing of three consecutive batches, which were manufactured as per Batch Manufacturing record for Export Market at Aurobindo Pharma Ltd. Unit-VII, Jadcherla. Samples were collected and analysed as per Process validation protocol. The data & test results of blend at various in-process phases were complied with the specified limits and final blend sample analysis results found to be complying within specifications. This study and results obtained assures that the manufacturing process is reproducible, yielding consistent product, which meets specification.

Key Words: Valacyclovir, Binding agents, Wetgranulatin, Dissolution Study, Validation Protocol.

ABSTRACT

Microspheres are characteristically free flowing powders consisting of proteins or synthetic polymers which are biodegradable in nature and ideally having a particle size less than 200 μm. The present study is aimed to formulate the Diclofenac Sodium (DFS) microspheres by using Co-acervation phase separation procedure to get better bioavailability of drug. In this study, we used DFS as a drug of choice since it has biological half-life of about 1-2 hrs. DFS microspheres were formulated by using different drug, gelatin and HPMC ratios. A total of 5 batches was F1, F2, F3, F4, F5 & F6 of varying concentrations of drug, gelatin and HPMC were prepared. DFS microspheres were evaluated by various tests like particle size analysis, angle of repose and bulk density. Further DFS Micro spheres were examined for in-vitro dissolution, by using phosphate buffer (PH 7.4) to make out the release of the drug.

Key words: Diclofenac Sodium, Micro spheres, Gelatin, Formaldehyde.

Abstract

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of Lamivudine in bulk and in pharmaceutical formulations. Lamivudine exhibits absorption maxima at 270 nm. Developed method obeyed the Beer’s law in the concentration range of 5 - 25 μg/mL. The method is accurate, precise and economical. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablet dosage forms. In this method, there is no interference from any common pharmaceutical additives and diluents. The % recovery is greater than 98 to101%. %, this shows that the method was free from the interference of excepients. The results of the tablet analysis were validated with respect to accuracy (recovery), linearity, limit of detection and limit of quantization were found to be satisfactory.

Keywords: UV Spectrophotometry, Lamivudine, Lamivudine Tablet.

ABSTRACT

The advanced methods of insulin delivery systems would gradually progress toward physiological insulin replacement and reduce the long-term complications of diabetes mellitus. Thus, a feasible alternative route for insulin delivery is likely to emerge in the future. This new millennium promises a revolutionary change in the delivery of insulin, which is not too far off for billions of sufferers who are reliant on subcutaneous administration. The approaches that seem to hold potential must be consolidated and converted to a working protocol. This review focused on various alternative delivery systems of insulin and each have their own set of favorable and unfavorable properties.

Keywords: Insulin delivery systems, Insulin, Diabetes mellitus, Hypoglycemia.

ABSTRACT

During the past few decades, numerous oral drug delivery systems have been developed to act as drug reservoirs from which the active substance can be released over a specific period of time at a predetermined and controlled rate. It is evident from the recent scientific and patent literatures that an increased interest in novel oral controlled release dosage forms that designed to be retained in the gastrointestinal tract (GIT) for a prolonged and predictable period of time exists today. Several approaches are currently utilized in the prolongation of the gastric residence times (GRT), including floating drug delivery systems (FDDS), low- density systems, raft systems incorporating alginate gels, bioadhesive or mucoadhesive systems, high-density systems, superporous hydrogels and magnetic systems. The current review focuses briefly about the FDDS that is one of the most leading methodologies in gastroretentive drug formulations.

Keywords: Floating drug delivery systems, Gastric residence time, Advantages.

ABSTRACT

Recombinant DNA is artificially created from two or more DNA incorporated into a single molecule. Genetic engineering, recombinant DNA technology, genetic modification/manipulation and gene splicing are terms that are applied to the direct manipulation of an organism‟s gene. Genetics is the science of genes, heredity, and the variation of organisms. In modern research, genetics provides important tools in the investigation of the function of a particular gene. The development of these new technologies have resulted into production of large amount of biochemically defined proteins of medical significance and created an enormous potential for pharmaceutical industries. The biochemically derived therapeutics is large extracellular proteins for use in either chronic replacement therapies or for the treatment of life threatening indications.

Keywords: Recombinant DNA, Therapeutics, Plasmids.

ABSTRACT

This present review is progress in selected nanotechnology topics and some possible applications. This attempt mainly focused on solid lipid nanoparticles & different preparation methods of solid lipid nanoparticles, nanostructured lipid carriers, lipid drug conjugates, route of administration and applications. For each of these, we review the underlying scientific concepts, potential enabling technologies and current limitations to the realization of nanotechnology applications.

Keywords: Nanotechnology, Solid lipid nanoparticles, Applications.